CytoFLEX LX Flow Cytometer

LSR Fortessa cell analyzer (BD Biosciences) uses up to five lasers (355 nm, 405 nm, 488 nm, 561 nm and 640 nm) that enable the detection of up to 18 colours simultaneously. The collection optics reducing excitation losses and improving light collection efficiency results in optical efficiency that delivers maximal sensitivity and resolution for multicolor applications. BD™ High Throughput Sampler (HTS) allows sample loading from 96-well microtiter plates for screening assays.

BD FACS Aria IIu cell sorter (BD Biosciences) is equipped with 3 lasers (405, 488, and 640 nm) and can detect up to 9 fluorescent parameters simultaneously. Four nozzle diameters are available (70 µm, 85 µm, 100 µm and 130 um). Applications include high speed, four-way cell sorting, cell purification, slide sorting, and cell cloning (by automated deposition into 6, 12, 24, 48 or 96 well plates). Samples as well as collected cells can be kept at desired temperatures (5 - 37 °C).

The CytoFLEX SRT cell sorter (Beckman Coulter) is equipped with a 4-laser system (405 nm, 488 nm, 561 nm and 638 nm) and allows detection in 15 fluorescence channels. Thanks to the interchangeable filter system, the instrument is able to measure the side scatter (SSC) from the 405 nm laser, which is suitable for analysis and subsequent sorting of smaller particles. A 100 μm nozzle and aerosol evacuation system is available. The instrument allows up to 4-way sorting into 15 ml and 5 ml tubes or separation into microtiter plates (up to 384-well plate).