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Late-stage labeling of diverse peptides and proteins with iodine-125
Journal of Pharmaceutical Analysis 15 (7): 101198 (2025)
The preparation of specifically iodine-125 (125I)-labeled peptides of high purity and specific activity represents a key tool for the detailed characterization of their binding properties in interaction with their binding partners. Early synthetic methods for the incorporation of iodine faced challenges such as harsh reaction conditions, the use of strong oxidants and low reproducibility. Herein, we review well-established radiolabeling strategies available to incorporate radionuclide into a protein of interest, and our long-term experience with a mild, simple and generally applicable technique of 125I late-stage-labeling of biomolecules using the Pierce iodination reagent for the direct solid-phase oxidation of radioactive iodide. General recommendations, tips, and details of optimized chromatographic conditions to isolate pure, specifically 125I-mono-labeled biomolecules are illustrated on a diverse series of (poly)peptides, ranging up to 7.6 kDa and 67 amino acids (aa). These…
Uncovering intramolecular singlet fission at the root of the dual fluorescence of 1,4-bis(p-nitro-β-styryl)benzene in solution
Chemical Science 2025: Early View
Milligram-Scale Enzymatic Synthesis of Base-Modified DNA through Primer Extension: Polymerase Synthesis and NMR Structures of DNA Containing Phenyl-Substituted Nucleobases
ChemistryEurope 2025: Early View
NMR-Derived Salt Bridges in Insulin Analogue: Resolving Artifactual Overbinding in Molecular Dynamics via Charge Scaling
Journal of Physical Chemistry Letters 16 (29): 7436–7442 (2025)