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Zwitterionic DNA: enzymatic synthesis of hypermodified DNA bearing four different cationic substituents at all four nucleobases
Nucleic Acids Research 53 (5): gkaf155 (2025)
We designed and synthesized a set of four 2′-deoxyribonucleoside 5′-O-triphosphates (dNTPs) bearing cationic substituents (protonated amino, methylamino, dimethylamino and trimethylammonium groups) attached to position 5 of pyrimidines or position 7 of 7-deazapurines through hex-1-ynyl or propargyl linker. These cationic dNTPs were studied as substrates in enzymatic synthesis of modified and hypermodified DNA using KOD XL DNA polymerase. In primer extension (PEX), we successfully obtained DNA containing one, two, three, or (all) four modified nucleotides, each bearing a different cationic modification. The cationic dNTPs were somewhat worse substrates compared to previously studied dNTPs bearing hydrophobic or anionic modifications, but the polymerase was still able to synthesize sequences up to 73 modified nucleotides. We also successfully combined one cationic modification with one anionic and two hydrophobic modifications in PEX. In polymerase chain reaction (PCR), we observed…
Computational metabolomics reveals overlooked chemodiversity of alkaloid scaffolds in Piper fimbriulatum
The Plant Journal 124 (5): e70086 (2025)
Liquid chromatography–inductively coupled plasma mass spectrometry analysis of peptides labelled with ClickZip mass tags
Analytica Chimica Acta 1350: 343853 (2025)
Acidobasic equilibria of inubosin derivatives studied by UV–Vis spectroscopy
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 334: 125950 (2025)