Procise 491 HT & 494 cLC Protein Sequencing Systems
The chemical process used in the Procise (Applied Biosystems) system is derived from the technique developed by Pehr Edman in the 1950’s for the sequential degradation of proteins and peptides. In the Edman degradation, a protein’s N-terminal amino acid is specifically reacted with phenylisothiocyanate (PITC). This derivatized aminoacid is then selectively removed, leaving the rest of the peptide chain intact. Each cycle of the degradation removes the new N-terminal amino acid from the peptide chain. The resulting PTHamino acids are analyzed sequentially (by RP HPLC) to determine the amino acid sequence of the protein or peptide. The IOCB (Drug Discovery Research Service Team) operates the Procise 491 HT Protein Sequencing System and the Procise 494 cLC Protein Sequencing System (high sensitivity analyses at sub-picomole level).
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