GC×GC-TOFMS LECO Pegasus BT 4D

Two dimensional capillary GC system, combined with the ultra-fast TOF-MS, changed our possibilities in analyses of complex samples of volatile compounds. No longer limited to two analytical dimensions, the Pegasus 4D GC×GC-TOFMS system (LECO, St. Joseph, Michigan, USA) provides us with four dimensions of analytical resolution for significantly more complete complex sample analysis. With Pegasus we are able to detect hundreds-to-thousands more volatile organic compounds than with previously used conventional one dimensional GC-MS. Separations, which previously resulted in dozens or hundreds of peaks, now yield thousands of individual components from complex mixtures. Other unique processing capabilities (such as sample comparisons) allow for automated mining of complex GC×GC data to extract previously unidentifiable similarities and differences.

The Agilent 7250 GC/Q-TOF system delivers full-spectrum, high-resolution, accurate mass data with a wide dynamic range for identifying and quantifying GC-amenable compounds. This high-resolution GC/Q-TOF enables accurate mass screening by GC/MS and enhanced compound identification through MS/MS, low-energy electron ionization, and complimentary chemical ionization techniques. The instrument is also equipped with direct inlet probe (by SIM). This supplement enables the direct acquisition of mass spectra without uncoupling the GC/MS interface.

In our lab, the instrument is mainly used for determination of elemental composition of volatile and semi-volatile organic compounds based on accurate mass measurement and for quantification in targeted metabolomic studies.

The Agilent 7250 GC/Q-TOF system was installed in December 2021.