Speaker
Prof. Ronald Micura (University of Innsbruck, Austria)
Topic
Synthetic modified RNA – Probes to Study Riboswitch and Ribosome Function
Series
Abstract
Our research aims at a comprehensive molecular understanding of important biological processes of nucleic acids, such as gene regulation by riboswitches and ribosomal translation. Chemical synthesis of modified RNA provides us with the necessary tools to achieve mechanistic insights into riboswitch function and ribosome catalysis.
References
- Haller, A.; Soulière, M. F.; Micura, R.The dynamic nature of RNA as key to understanding riboswitch mechanisms. Acc. Chem. Res. 2011, 44, 1339-1348.
- Haller, A.; Rieder, U.; Aigner M.; Blanchard, S. C.; Micura, R. Conformational capture of the SAM-II riboswitch. Nat. Chem. Biol. 2011, 7, 393-400.
- Santner, T.; Rieder, U.; Kreutz, C.; Micura, R. Pseudoknot preorganization of the preQ1 class I riboswitch. J. Am. Chem. Soc. 2012, in revision.
- Rieder, U.; Kreutz, C.; Micura, R. Folding of a transcriptionally acting preQ1 riboswitch. Proc. Natl. Acad. Sci. USA. 2010, 107, 10804-10809.
- Geiermann, A.-S.; Polacek, N.; Micura, R. Native chemical ligation of hydrolysis-resistant 3’-peptidyl-tRNA mimics. J. Am. Chem. Soc. 2011, 133, 19068-19071.
- Graber, D.; Moroder, H.; Steger, J.; Trappl, K.; Polacek, N., Micura, R. Efficient semisynthesis of hydrolysis-resistant 3'-peptidyl-tRNA conjugates containing genuine tRNA modifications. Nucl. Acids Res. 2010, 38, 6796-6802.
- Moroder, H.; Steger, J.; Graber, D.; Fauster, K.; Trappl, K.; Marquez, V.; Polacek, N.; Wilson, D. N.; Micura, R. Nonhydrolyzable RNA–peptide conjugates: A powerful advance in the synthesis of mimics for 3'-peptidyl tRNA termini. Angew. Chem. Int. Ed. 2009, 48, 4056-4060; Angew. Chem. 2009, 121, 4116-4120.
- Lang, K.; Erlacher, M.; Wilson, D. N.; Micura, R.; Polacek, N. The role of 23S rRNA residue A2451 in ribosomal peptide bond synthesis revealed by atomic mutagenesis. Chem. Biol. 2008, 15, 485-492.
- Chirkova, A.; Erlacher, M.; Micura, R.; Polacek, N. Chemically engineered ribosomes: A new frontier in synthetic biology. Curr. Org. Chem. 2010, 14, 148-161.
- Fauster, K.; Hartl, M.; Santner, T.; Aigner, M.; Kreutz, C.; Bister, K.; Ennifar, E.; Micura, R. 2- Azido RNA, a versatile tool for chemical biology: Synthesis, X-ray structure, siRNA applications, click labeling. ACS Chem. Biol. 2012, 7, 581-589.
